Like a blazing comet, I've traversed infinite nights, interstellar spaces of the imagination, voluptuousness and fear.
The factor most ignored in discussing interstellar flight is the kinetic energy that must be invested in the ship to make its tons of matter move at a substantial fraction of the speed of light.
What if life is not carbon-based? Can life exist as a gas or a plasma? Could planets or stars in some sense be alive? What about an interstellar cloud? Could life exist on such a small or large scale, or move so fast or so slowly that we wouldn't recognize it? Could you have an intelligent virus?
Lots of science fiction deals with distant times and places. Intrepid prospectors in the Asteroid Belt. Interstellar epics. Galactic empires. Trips to the remote past or future.
Human uploads have such a natural advantage over present-day people in the environment of space, it's exceedingly unlikely flesh-and-blood beings will ever engage in interstellar travel.
We don't know how to identify intelligence over interstellar distances, so what we do instead is use technology for a proxy.