Jack's Father: Who are you?
Spider-Man: Spider-Man.
Ben Parker: [to Gwen] He's got you on his computer. I'm his probation officer.
George Stacy: Mayor, if there was a large dinosaur running around New York, you would be the first to know.
Miss Ritter: I had a professor once who liked to tell his students that there were only 10 different plots in all of fiction. Well, I'm here to tell you he was wrong. There is only one: "Who am I?"
May Parker: Peter, listen to me. Secrets have a cost. They're not free. Not now, not ever.
[from trailer]
Gwen Stacy: You're a wanted man, Peter Parker.
Spider-Man: [while The Lizard is slamming him against the walls] Don't... make me... have to... hurt you!
Gwen Stacy: Where you headed?
Peter Parker: Uh... Monday. B track.
Gwen Stacy: Oh, it's Thursday.
May Parker: Ben Parker, don't you even think about leaving that filthy box in my kitchen!
Ben Parker: These are my bowling trophies.
May Parker: [sarcastically] Oh, but then, by all means, please leave that filthy box in my kitchen.
George Stacy: So 38 of New York's finest versus one guy in a unitard. Am I correct?
Dr. Curt Connors: Did you know there's rumor of a new species in New York? Beautiful but quite large.
Peter Parker: What do you know about it? Have you seen it?
Dr. Curt Connors: No, it's not yet classified. But it can be aggressive... if threatened.
Peter Parker: [after getting beaten up by Flash for not taking the picture] I'm still not taking the picture.
Dr. Curt Connors: I'm sure you're a very nice young man, but this is a home. Make an appointment with my office.
Peter Parker: Dr Connors? I'm Richard Parker's son.
Dr. Curt Connors: Peter?
The Lizard: All these souls, lost and alone. I can save them. I can cure them. There's no reason to stop me, Peter.
Dr. Curt Connors: I spent my life as a scientist trying to create a world without weakness, without outcasts. I sought to create a stronger human being, but there's no such thing. Human beings are weak, pathetic, feeble-minded creatures. Why be a human at all when we can be so much more? Faster, stronger, smarter. This is my gift to you.
[Peter finds an old satchel in a closet]
Ben Parker: I forgot all about that thing. It was your dad's.
Ben Parker: [as Peter tends to his injured face] How's the other guy look?
[Peter looks at him in confusion]
Ben Parker: Come on. You think I don't know a right cross when I see one? Yes or no: do I need to call somebody's father?
Dr. Curt Connors: Welcome. My name is Dr. Curtis Connors. And yes, in case you're wondering, I'm a southpaw. I'm not a cripple, I'm a scientist, and I am the world's most foremost authority in herpetology. That's reptiles, for those of you who don't know.
Ben Parker: I think it's the condenser tray.
Peter Parker: No, too much water for the condenser tray or the heat exchange tubing. This has got to be the fill line.
Ben Parker: That's the only thing that makes any sense...
